The Anti-Insulin ELISA by ORGENTEC is an indirect enzyme immunoassay for the quanti- tative detection of antibodies against animal (cow, pig) and human insulin. Primarily, this test is used for the detection of autoantibodies against human insulin for the early diagnosis of type I diabetes.
Autoantibodies against insulin can appear in the prediabetic phase of type I diabetes mellitus or upon its manifestation. These autoantibodies are found in 50 to 70 % of children with diabetes mellitus and in 20 to 30 % of affected adults. In rare cases, insulin-dependent diabetes patients generate antibodies against exogenous insulin. These antibodies can give rise to insulin-resistance and should therefore be controlled when monitoring insulin therapy.
The wells of the Anti-Insulin ELISA by ORGEN- TEC are coated with a mixture of cow, pig and recombinant human insulin. |
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| Tests |
12 × 8 determinations
12 or 24 Alegria® test strips |
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| Calculation |
quantitative |
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| Range |
0-100 U/ml |
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| Cut-off |
10 U/ml |
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| Sensitivity |
0.5 U/ml |
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| Calibration |
arbitrary |
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| Substrate |
TMB (450 nm) |
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| Antigens |
Insulin (bovine, porcine,
human recombinant) |
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Instruction for Use: ORG 520-0802-02-gb.pdf
Material-Safety-Datasheet: ORG 520-msds-v3.pdf