Liver-9-Line 2nd Generation

The Liver-9-Line 2nd Generation immunoblot test is a membrane-based enzyme immunoassay for the semi-quantitative determination of IgG autoantibodies against nine test antigens in human serum or plasma. It is used in cases of suspected autoimmune liver disease such as type 1 or type 2 autoimmune hepatitis (AIH-1, AIH-2), primary biliary cirrhosis (PBC), and primary sclerosing cholangitis (PSC).
In particular, the simultaneous detection of autoantibodies against specific marker antigens makes it possible to differentiate between type 1 and type 2 AIH and to rule out other liver diseases.
Elevated titers of antinuclear antibodies (ANA) and/or anti-smooth muscle antibodies (ASMA) are typical of AIH-1. With the Liver-9-Line 2nd Generation test, detection of antibodies against the individual smooth-muscle antigens F-Actin, alpha-actinin, and tropomyosin serves to differentiate this disease. The analysis of these diagnostically relevant biomarkers also allows for assessment of disease activity and evaluation of further progression. Autoantibodies against soluble liver antigen (SLA), also known as liver-pancreas antigen (LP) are also highly specific markers for AIH-1.
Antibodies against liver-kidney microsome type 1 (LKM-1) and liver cytosolic protein type 1 (LC1) are characteristic of AIH-2. They are indicative of severe progression and LC1 antibody titers correlate with disease activity.
Anti-mitochondrial antibodies of the M2 subtype (AMA-M2) indicate PBC. They are absent in up to 5 % of PBC patients. In such cases, the highly specific antibodies against nuclear body antigen Sp100 and nuclear pore antigen gp210 are diagnostically important.

Automated evaluation of immunoblots with iVISION Scanware

iVISION Scanware makes it possible to automate the evaluation of immunoblots. This software allows for the objective assessment of laboratory results and efficiently stores and archives the data.

Specifications

Product Number ORG 722
Tests8 strips / ORG 722-08, 16 strips / ORG 722-16
Calibrationinternal
Calculationsemi-quantitative
Coating alpha-actinin, F-actin, nuclear pore antigen gp210, LC1, LKM-1 (recombinant) , M2 proteins (PDC-E2, BCOADC-E2, OGDC-E2), SLA/LP, nuclear body antigen Sp100, tropomyosin
Alegria

ORG 256   ORG 253   ORG 216   ORG 257   ORG 254  

ELISA

ORG 516  

BLOT

ORG 760  

Documentation

Instruction for Use
Safety Data Sheet
Product Information