Gastro-5-Line

The Gastro-5-Line immunoblot test is a membrane-based enzyme immunoassay for the semi-quantitative determination of IgG and IgA autoantibodies in human serum or plasma when autoimmune disease of the gastrointestinal tract is suspected.
Gastro-5-Line is intended for screening and for the differential diagnosis of Crohn’s disease, ulcerative colitis, celiac disease (sprue), and pernicious anemia.
Each nitrocellulose strip is coated with the antigens most commonly found in cases of gastrointestinal autoimmune disease:
Antibodies against mannan from Saccharomyces cerevisiae (ASCA) are ideal for the differentiation of Crohn’s disease and ulcerative colitis.
Anti-tissue transglutaminase is a reliable marker for celiac disease. In addition, celiac patients also have serum antibodies against gliadin. Celiac patients often form no IgA antibodies. For this reason, the Gastro-5-Line immunoblot simultaneously detects both IgG and IgA class antibodies.
Antibodies against intrinsic factor are found in patients with pernicious anemia. Because of its high sensitivity, parietal cell antigen is a good screening parameter for pernicious anemia.

Automated evaluation of immunoblots with iVISION Scanware

iVISION Scanware makes it possible to automate the evaluation of immunoblots. This software allows for the objective assessment of laboratory results and efficiently stores and archives the data.

Specifications

Product Number ORG 740
Tests8 strips / ORG 740-08, 16 strips / ORG 740-16
Calibrationinternal
Calculationsemi-quantitative
Coating gliadin, H+/K+-ATPase from parietal cells, intrinsic factor (human recombinant), mannan (Saccharomyces cerevisiae), tissue transglutaminase (tTG) (human recombinant)

Documentation

Instruction for Use
Safety Data Sheet