Nucleo-9-Line

The Nucleo-9-Line test is a membrane-based enzyme immunoassay for the semi-quantitative determination of IgG autoantibodies against various nuclear antigens (ANA) in human serum or plasma.
The immunoblot Nucleo-9-Line uses nine antigens that typically appear in various inflammatory rheumatologic conditions. Especially Sm, dsDNA and nucleosomes play an important role in the diagnosis of systemic lupus erythematosus (SLE). The nine purified antigens are individually applied as bands on a nitrocellulose strip. For detection bound antibodies are stained by an enzyme-catalysed substrate reaction.
The Nucleo-9-Line blot is an important and easy-to-use test system for the further differentiation of ANA after ELISA screening or immunofluorescence tests.

Automated evaluation of immunoblots with iVISION Scanware

iVISION Scanware makes it possible to automate the evaluation of immunoblots. This software allows for the objective assessment of laboratory results and efficiently stores and archives the data.

Specifications

Product Number ORG 711
Tests8 strips / ORG 711-08 , 16strips / ORG 711-16
CalibrationCDC sera
Calculationsemi-quantitative
Coating centromere B, dsDNA (recombinant), histidyl-tRNA synthetase (Jo-1), nucleosomes (human), RNP/Sm complex, Scl-70 protein (DNA topoisomerase I), Sm ribonucleoproteins, SS-A 52 (Ro 52), SS-A 60 (Ro 60), SS-B (La)
Alegria

ORG 200   ORG 233   ORG 238   ORG 204A   ORG 204G   ORG 204M   ORG 204S   ORG 206   ORG 213   ORG 228   ORG 211   ORG 212   ORG 210   ORG 208   ORG 252   ORG 260   ORG 209  

ELISA

ORG 538   ORG 539   ORG 600   ORG 633   ORG 604   ORG 604A   ORG 604M   ORG 604S   ORG 506   ORG 513   ORG 514   ORG 546   ORG 528   ORG 511   ORG 544   ORG 512   ORG 510   ORG 508   ORG 652   ORG 660   ORG 509  

BLOT

ORG 710   ORG 760  

Documentation

Instruction for Use
Safety Data Sheet