Anti-nDNA Antibodies (nDNA)

Indirect immunofluorescence test for the qualitative determination of anti-nDNA IgG antibodies in serum or plasma.

Detection of antibodies against native double-stranded DNA (Anti-nDNA, Anti-dsDNA) is very important for laboratory diagnostics of systemic lupus erythematosous (SLE). Immunofluorescence assays based on antigens of the flagellate Crithidia luciliae have a high diagnostic specificity. Sensitivity is somewhat lower, especially in patients with no signs for an active disease state.
Antibodies against native DNA are more than specific markers for the disease; they are also suitable parameters for measuring lupus disease activity. Therefore, Anti-nDNA antibodies are among the most frequently detected autoantibodies that are associated with SLE. They are found in 95 % of SLE patients with renal involvement, in 50 – 70 % of SLE patients without renal involvement and in 40 % of patients with inactive SLE. Anti-nDNA antibodies are rarely found in healthy individuals.
Each component of the complete Anti-nDNA kit (ORG 870) can be ordered separately.

Specifications

Product Number ORG 871
Tests 120 (10 slides, 12 wells each)
Coating Crithidia luciliae

Documentation

Instruction for Use
Safety Data Sheet
IFA Components

Components of the kit

Anti-nDNA Antibodies (nDNA)ORG 801
Crithidia luciliae – 10 slides x 12 wells
nDNA Positive ControlORG 835
anti-nDNA antibodies (for Crithidia luciliae) – 0.3 ml
IgG FITC/EvansORG 837
for human cells and Crithidia luciliae – 3 ml
Mounting MediumORG 842
mounting medium – 3 ml
PBS (10x)ORG 843
phosphate buffer saline (PBS 10x) – 100 ml
Negative ControlORG 844
Negative Control for IFA – 0.3 ml
Blotting Paper 1 x 12ORG 845
filter paper for 12 wells – 10 pcs.