Autoantibodies RL/RK/RS

Indirect immunofluorescence test for the qualitative determination of autoimmune antibodies in serum or plasma.

Sections of rat liver, kidney (Cortex and medulla), and stomach are the substrate on the slide. They detect autoantibodies in serum or plasma against the cell nucleus (ANA) the mitochondria (AMA), smooth muscle antigens (ASMA), liver-kidney microsomes, reticulin and other structures.
The different types of antibodies are associated with characteristic fluorescence patterns:
ANA – Homogeneous fluorescence of the nucleus of interphase cells, bright fluorescence of mitotic cells.
AMA – Granular fluorescence of mitochondria in the cytoplasm of hepatocytes and renal tubular cells.
ASMA – Staining of the muscle layers of the blood vessels and the interglandular fibers in rat stomach.
APCA – Intracellular staining of parietal cells in the rat gastric mucosa.
LKM-1 – Bright staining of the cytoplasm of the hepatocytes in the liver and the in the proximal tubules of the kidney. No staining of the distal tubules.
In SLE patients the sensitivity of ANA determination is higher than 95 %. The presence of mitochondrial antibodies is associated with primary biliary cirrhosis in more than 95 % of patients. Anti-smooth muscle antibodies are found in the sera of 52-85 % of patients with chronic autoimmune hepatitis and 22 % of patients with primary biliary cirrhosis. Antibodies against gastric parietal cells are found in 90 % of patients with pernicious anaemia. Anti-LKM type I antibodies are specific markers for autoimmune hepatitis type II.
Controls for different fluorescence patterns – speckled (ORG 828, ANA-Sp), nucleolar (ORG827, ANA-Nu) or APCA (ORG 829, APCA) are available and can be purchased separately in addition to the RL/RK/RS kit. Each component of the complete kit ORG 874 can be ordered separately.

Specifications

Product Number ORG 874
Tests 96 (12 slides, 8 wells each)
Coating rat liver, rat stomach, rat kidney

Documentation

Instruction for Use
Safety Data Sheet
IFA Components

Components of the kit

Autoantibodies RL/RK/RSORG 804
rat liver / kidney (cortex + medulla) / stomach – 12 slides x 8 wells
AMA Positive ControlORG 823
anti-mitochondrial antibodies – 0.3 ml
ANA-RL Positive ControlORG 826
anti-nuclear antibodies (rat liver) – 0.3 ml
ASMA Positive ControlORG 833
anti-smooth muscle antibodies – 0.3 ml
FITC/Evans (R)ORG 839
IgG / IgA / IgM, for rat and mouse tissue – 2.5 ml
Mounting MediumORG 842
mounting medium – 3 ml
PBS (10x)ORG 843
phosphate buffer saline (PBS 10x) – 100 ml
Negative ControlORG 844
Negative Control for IFA – 0.3 ml
Blotting Paper 1 x 8ORG 846
filter paper for 8 wells – 12 pcs.

Auxiliary reagents

ANA-Nucleolar Positive ControlORG 827
anti-nuclear antibodies (“nucleolar pattern”) – 0.3 ml
ANA-Speckled Positive ControlORG 828
anti-nuclear antibodies (“speckled pattern”) – 0.3 ml
APCA Positive ControlORG 829
anti-parietal cell antibodies – 0.3 ml