Anti-Neutrophil Cytoplasmic Antibodies (ANCA-Ethanol)

Indirect immunofluorescence test for the qualitative determination of anti-neutrophil cytoplasmic IgG antibodies (ANCA) in serum or plasma on ethanol-fixed human neutrophil granulocytes.

Autoantibodies against cytoplasmic antigens of neutrophil granulocytes (ANCA) are acknowledged serological markers for the diagnosis of systemic vasculitis. Our slides contain ethanol-fixed granulocytes, which facilitate the differentiation of granulomatosis with polyangiitis (GPA; formerly Wegener’s granulomatosis) from eosinophilic granulomatosis with polyangiitis (EGPA; formerly Churg-Strauss syndrome), microscopic polyangiitis (MPA), or Goodpasture syndrome.
A granular immunofluorescence pattern in the cytosol (cANCA) indicates antibodies against proteinase 3 (PR3), which are indicative of GPA. A dense immunofluorescence pattern around the cell nucleus (pANCA) denotes antibodies against myeloperoxidase (MPO), which occur in cases of EGPA or MPA. Our formalin-fixed granulocyte test ORG 892 should be used subsequent to a pANCA positive test result with ethanol-fixed granulocytes to differentiate a true pANCA pattern from an ANA result with a similar perinuclear fluorescence pattern.
In accordance with the “ANCA Consensus Statement” we recommend further serological diagnostic an antigen-specific ELISA test for the individual detection of anti-PR3 (ORG 618, ORG 318) and anti-MPO (ORG 519, ORG 219).

Specifications

Product Number ORG 891
Tests60 (10 slides, 6 wells each)
Coating ethanol fixed neutral granulocytes

Documentation

Instruction for Use
Safety Data Sheet
Product Information
IFA Components

Components of the kit

IgG FITC/EvansORG 837
for human cells and Crithidia luciliae – 3 ml
Mounting MediumORG 842
mounting medium – 3 ml
PBS (10x)ORG 843
phosphate buffer saline (PBS 10x) – 100 ml
Negative ControlORG 844
Negative Control for IFA – 0.3 ml
C-ANCA Positive ControlORG 889
antibodies against proteinase 3 – 0.3 ml
P-ANCA Positive ControlORG 890
antibodies against myeloperoxidase – 0.3 ml
Anti-Neutrophil Cytoplasmic Antibodies (ANCA-Ethanol)ORG 893
ethanol-fixed human neutrophil granulocytes – 10 slides x 6 wells
Blotting Paper 1 x 6ORG 895
filter paper for 6 wells – 10 pcs.