Anti-MPO (pANCA)

Anti-MPO is an ELISA test for the quantitative determination of IgG antibodies against myeloperoxidase (MPO) in human serum or plasma.

The anti-MPO assay is an important element in the diagnosis of microscopic polyangiitis (MPA). MPO antibodies are also found in patients with eosinophilic granulomatosis with polyangiitis (EGPA, Churg-Strauss syndrome) and polyarteritis nodosa. They can be used to serologically differentiate these diseases from granulomatosis with polyangiitis (GPA, formerly Wegener’s disease). Antineutrophil cytoplasmic antibodies (ANCA) are a group of autoantibodies directed against the cytoplasmic components of the neutrophils and monocytes. Current recommendations for ANCA detection include initial testing by indirect immunofluorescence (IF), followed by an antigen-specific ELISA such as Anti-MPO. MPO is a lysosomal enzyme of neutrophil granulocytes and monocytes. Antibodies against MPO exclusively recognize native conformational epitopes. The wells of the Anti-MPO ELISA are coated with highly purified human myeloperoxidase that fully retains its native conformation.

Specifications

Product Number ORG 519
Tests12 x 8 tests
Calibrationinternal
Calculationquantitative
Range0 - 100 U/ml
Cut-off 5 U/ml
Coating myeloperoxidase (MPO)
Alegria

ORG 219   ORG 389  

ELISA

ORG 530   ORG 689  

BLOT

ORG 789  

Documentation

Instruction for Use
Safety Data Sheet

Document Archive
Instruction for Use Revision 3. Earlier versions and additional languages can be found in the document archive.