Anti-Smooth Muscle Antibodies (ASMA)

Indirect immunofluorescence test for the qualitative determination of antibodies against smooth muscle (ASMA) in serum or plasma.

Tissue sections from rat stomach are used for the detection of anti-smooth muscle antibodies (ASMA) in serum or plasma. ASMA bind to the corresponding antigens in the muscle layers of the blood vessels and the interglandular fibrils in the rat stomach. Anti-smooth muscle antibodies are the standard diagnostic markers for autoimmune hepatitis. Anti-actin antibodies, a subgroup of smooth muscle antibodies, are found in the sera of 52-85 % of patients with active chronic autoimmune hepatitis and 22 % of patients with primary biliary cirrhosis. Their presence in other autoimmune diseases, like cholangyopathy, reflects overlapping features of those diseases.


Product Number ORG 877
Tests 96 (12 slides, 8 wells each)
Coating rat stomach


Instruction for Use
Safety Data Sheet
IFA Components
Instruction for Use Revision 2. Earlier versions and additional languages can be found in the document archive.

Components of the kit

ASMA Positive ControlORG 833
anti-smooth muscle antibodies – 0.3 ml
FITC/Evans (R)ORG 839
IgG / IgA / IgM, for rat and mouse tissue – 2.5 ml
Mounting MediumORG 842
mounting medium – 3 ml
PBS (10x)ORG 843
phosphate buffer saline (PBS 10x) – 100 ml
Negative ControlORG 844
Negative Control for IFA – 0.3 ml
Blotting Paper 1 x 8ORG 846
filter paper for 8 wells – 12 pcs.
Anti-Smooth Muscle Antibodies (ASMA)
rat stomach – 12 slides x 8 wells
available with kit ORG 877 only
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