Anti-Smooth Muscle Antibodies (ASMA)
Indirect immunofluorescence test for the qualitative determination of antibodies against smooth muscle (ASMA) in serum or plasma.
Tissue sections from rat stomach are used for the detection of anti-smooth muscle antibodies (ASMA) in serum or plasma. ASMA bind to the corresponding antigens in the muscle layers of the blood vessels and the interglandular fibrils in the rat stomach.
Anti-smooth muscle antibodies are the standard diagnostic markers for autoimmune hepatitis. Anti-actin antibodies, a subgroup of smooth muscle antibodies, are found in the sera of 52-85 % of patients with active chronic autoimmune hepatitis and 22 % of patients with primary biliary cirrhosis. Their presence in other autoimmune diseases, like cholangyopathy, reflects overlapping features of those diseases.
Control, conjugate, reagents and consumables that are components of the complete ASMA kit ORG 877 are individually available and can be reordered separately.
Specifications
| Product Number |
ORG 877 |
| Tests | 96 (12 slides, 8 wells each) |
| Coating |
rat stomach |
|
|
|
Instruction for Use Revision 2. Earlier versions and additional languages can be found in the document archive.
|
Components of the kit
| ASMA Positive Control | ORG 833 |
| anti-smooth muscle antibodies – 0.3 ml |
| FITC/Evans (R) | ORG 839 |
| IgG / IgA / IgM, for rat and mouse tissue – 2.5 ml |
| Mounting Medium | ORG 842 |
| mounting medium – 3 ml |
| PBS (10x) | ORG 843 |
| phosphate buffer saline (PBS 10x) – 100 ml |
| Negative Control | ORG 844 |
| Negative Control for IFA – 0.3 ml |
| Blotting Paper 1 x 8 | ORG 846 |
| filter paper for 8 wells – 12 pcs. |
| Anti-Smooth Muscle Antibodies (ASMA) | |
rat stomach – 12 slides x 8 wells
available with kit ORG 877 only |
