Anti-Neutrophil Cytoplasmic Antibodies (ANCA-Ethanol)

Indirect immunofluorescence test for the qualitative determination of anti-neutrophil cytoplasmic IgG antibodies (ANCA) in serum or plasma on ethanol-fixed human neutrophil granulocytes.

Autoantibodies against cytoplasmic antigens of neutrophil granulocytes (ANCA) are acknowledged serological markers for the diagnosis of systemic vasculitis. Our slides contain ethanol-fixed granulocytes, which facilitate the differentiation of granulomatosis with polyangiitis (GPA; formerly Wegener’s granulomatosis) from eosinophilic granulomatosis with polyangiitis (EGPA; formerly Churg-Strauss syndrome), microscopic polyangiitis (MPA), or Goodpasture syndrome.
A granular immunofluorescence pattern in the cytosol (cANCA) indicates antibodies against proteinase 3 (PR3), which are indicative of GPA. A dense immunofluorescence pattern around the cell nucleus (pANCA) denotes antibodies against myeloperoxidase (MPO), which occur in cases of EGPA or MPA. Our formalin-fixed granulocyte test ORG 892 should be used subsequent to a pANCA positive test result with ethanol-fixed granulocytes to differentiate a true pANCA pattern from an ANA result with a similar perinuclear fluorescence pattern.
In accordance with the “ANCA Consensus Statement” we recommend further serological diagnostic an antigen-specific ELISA test for the individual detection of anti-PR3 (ORG 618, ORG 318) and anti-MPO (ORG 519, ORG 219).

Specifications

Product Number ORG 891
Tests60 (10 slides, 6 wells each)
Coating ethanol fixed neutral granulocytes

Documentation

Instruction for Use
Safety Data Sheet
Product Information
IFA Components
Instruction for Use Revision 2. Earlier versions and additional languages can be found in the document archive.

Components of the kit

IgG FITC/EvansORG 837
for human cells and Crithidia luciliae – 3 ml
Mounting MediumORG 842
mounting medium – 3 ml
PBS (10x)ORG 843
phosphate buffer saline (PBS 10x) – 100 ml
Negative ControlORG 844
Negative Control for IFA – 0.3 ml
C-ANCA Positive ControlORG 889
antibodies against proteinase 3 – 0.3 ml
P-ANCA Positive ControlORG 890
antibodies against myeloperoxidase – 0.3 ml
Anti-Neutrophil Cytoplasmic Antibodies (ANCA-Ethanol)ORG 893
ethanol-fixed human neutrophil granulocytes – 10 slides x 6 wells
Blotting Paper 1 x 6ORG 895
filter paper for 6 wells – 10 pcs.
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