Liver Disease Diagnostics by Immunoblot
Mainz, 16/05/2011 – Mainz based ORGENTEC Diagnostika GmbH has developed a new immunoblot test for the serological diagnosis of autoimmune liver disease.
Liver-9-Line facilitates the rapid and cost-effective diagnosis of autoimmune hepatitis (type 1 and type 2 AIH) and primary biliary cirrhosis (PBC) in a single step. The test system also allows for the reliable differentiation of these autoimmune diseases from other diseases of the liver. This immunoblot is suitable for initial and differential diagnosis in cases of ambiguous symptomatology or for differentiation of a positive result from screening carried out with ELISA or immunofluorescence tests.
The comprehensive set of antigens included in this new immunoblot is unique on the market: In addition to the three ASMAs, F-actin, myosin, and desmin, Liver-9-Line also detects antibodies against soluble liver antigen (SLA/LP), liver kidney microsomal type 1 (LKM-1), and liver cytosol antigen type 1 (LC1). The PBC-typical autoantibodies detected by this immunoblot include AMA-M2 as well as antibodies against Sp100 and gp210.
The analytical software from ORGENTEC Diagnostika allows for the automated evaluation of the immunoblots. The iVISION Scanware makes it possible to objectively analyze and securely archive test data.
Overview of the antigens used in the Liver-9-Line test: M2, Sp100, gp210, SLA/LP, LKM-1, LC1, F-actin, desmin, myosin. Liver-9-Line is available in packages of 8 or 16 immunoblot strips, all reagents are ready for immediate use. Each individual blot strip includes serum, conjugate control, and cut-off control, as well as an individual code that eliminates accidental mix-ups.