Still the various immunofluorescence assays carry great weight for modern medicine, both in research and in medical diagnostics. Along these lines, indirect immunofluorescence assays, or for short: IIFA or IFA, are the gold standard for many issues of serodiagnosing autoimmune diseases.

Numerous images of typical immunofluorescence patterns

Experience and expertise of the personnel remain important criteria for the high quality of autoimmunology findings. It is the purpose of this immunofluorescence atlas to bring together the necessary knowledge and experience in reporting test results and to make this knowledge available the easy and straightforward way. To this effect, we place the e-book of our Immunofluorescence Guide for autoimmune disease diagnostics on this web site at your free disposal:

Please click on the preview to open ebook.

Whether it is the immunofluorescence pattern of antinuclear antibodies in HEp-2 cells assays or it is autoantibodies detection on tissue sections from rats: our Immunofluorescence Guide for autoimmune diagnostics features carefully selected and splendid photographs of typical immunofluorescence patterns of common autoantibodies. Compact notes provide useful indications how test results have to be interpreted. Authoress of this guide is Barbara Fabian from the GRÜNER CLUB AUTOIMMUN.

IFA for high-quality diagnostics in autoimmunity labs

In the early 1940s the American physician and immunology pioneer Albert Hewett Coons, M.D. was the first person who conceptualized and developed immunofluorescent techniques for labelling antibodies. Since that time various immunofluorescence methods have been continually improved and methodically refined. Still the use of indirect immunofluorescence allows for cost-effective and high-quality diagnosis in the autoimmunity laboratory. Positive IFA results are subsequently further clarified with other immunological tests, including ELISA and immunoblots, in a stepwise diagnostic process.

For this purpose ORGENTEC’s ELISA and immunoblot test systems provide some of the most modern and most useful solution for autoimmunity diagnostics. Innovative analysis systems as for instance the Alegria^® instrument allow for fully automated processing of ELISA test systems. Concerning this the online Immunofluorescence Guide provides helpful hints, too.