ENAscreen
ENAscreen is an ELISA-based screening test for the qualitative determination of IgG antibodies against components of the cell nucleus in human serum or plasma.
Besides DNA and histones, autoantibodies may also target other nuclear antigens. These nuclear antigens were named extractable nuclear antigens (ENA), as originally they were extracted from the nuclei with saline solution. Autoantibodies to Smith antigen (Sm) which is also considered to be highly specific for SLE were the first anti-ENA detected. Thereafter, further subtypes of ENA i.e. ribonucleoproteins (RNP), the Sjoegren antigens SS-A and SS-B, Scl-70, and Jo-1 were identified.
Each individual well of the ENAscreen test is coated with a mixture of six highly purified antigens. For further differentiation, a positive result with this ELISA may then be followed up with ENAcombi or the ORGENTEC test kits for quantitative determination of the individual ENA specificities.
Specifications
Product Number |
ORG 206 |
Tests | 24 Alegria® strips |
Calibration | CDC sera |
Calculation | qualitative |
Cut-off | Index > 1.0 |
Coating |
histidyl-tRNA synthetase (Jo-1), RNP/Sm complex, Scl-70 protein (DNA topoisomerase I), Sm ribonucleoproteins, SS-A 52 (Ro 52), SS-A 60 (Ro 60), SS-B (La) |
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Instruction for Use Revision 5. Earlier versions and additional languages can be found in the document archive.
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