Anti-nDNA Antibodies (nDNA)
Indirect immunofluorescence test for the qualitative determination of anti-nDNA IgG antibodies in serum or plasma.
Detection of antibodies against native double-stranded DNA (Anti-nDNA, Anti-dsDNA) is very important for laboratory diagnostics of systemic lupus erythematosous (SLE). Immunofluorescence assays based on antigens of the flagellate Crithidia luciliae have a high diagnostic specificity. Sensitivity is somewhat lower, especially in patients with no signs for an active disease state.
Antibodies against native DNA are more than specific markers for the disease; they are also suitable parameters for measuring lupus disease activity. Therefore, Anti-nDNA antibodies are among the most frequently detected autoantibodies that are associated with SLE. They are found in 95 % of SLE patients with renal involvement, in 50 – 70 % of SLE patients without renal involvement and in 40 % of patients with inactive SLE. Anti-nDNA antibodies are rarely found in healthy individuals.
Specifications
| Product Number |
ORG 871 |
| Tests | 120 (10 slides, 12 wells each) |
| Coating |
Crithidia luciliae |
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Instruction for Use Revision 2. Earlier versions and additional languages can be found in the document archive.
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Components of the kit
| Slides Anti-nDNA Antibodies (nDNA) | |
| Crithidia luciliae – 10 slides x 12 wells |
| nDNA Positive Control | |
| anti-nDNA antibodies (for Crithidia luciliae) – 0.3 ml |
| IgG FITC/Evans | |
| for human cells and Crithidia luciliae – 3 ml |
| Mounting Medium | |
| mounting medium – 3 ml |
| PBS (10x) | |
| phosphate buffer saline (PBS 10x) – 100 ml |
| Negative Control | |
| Negative Control for IFA – 0.3 ml |
| Blotting Paper 1 x 12 | |
| filter paper for 12 wells – 10 pcs. |
