Anti-Neutrophil Cytoplasmic Antibodies (ANCA-Ethanol)

Indirect immunofluorescence test for the qualitative determination of anti-neutrophil cytoplasmic IgG antibodies (ANCA) in serum or plasma on ethanol-fixed human neutrophil granulocytes.

Autoantibodies against cytoplasmic antigens of neutrophil granulocytes (ANCA) are acknowledged serological markers for the diagnosis of systemic vasculitis. Our slides contain ethanol-fixed granulocytes, which facilitate the differentiation of granulomatosis with polyangiitis (GPA; formerly Wegener’s granulomatosis) from eosinophilic granulomatosis with polyangiitis (EGPA; formerly Churg-Strauss syndrome), microscopic polyangiitis (MPA), or Goodpasture syndrome.
A granular immunofluorescence pattern in the cytosol (cANCA) indicates antibodies against proteinase 3 (PR3), which are indicative of GPA. A dense immunofluorescence pattern around the cell nucleus (pANCA) denotes antibodies against myeloperoxidase (MPO), which occur in cases of EGPA or MPA. Our formalin-fixed granulocyte test ORG 892 should be used subsequent to a pANCA positive test result with ethanol-fixed granulocytes to differentiate a true pANCA pattern from an ANA result with a similar perinuclear fluorescence pattern.
In accordance with the “ANCA Consensus Statement” we recommend further serological diagnostic an antigen-specific ELISA test for the individual detection of anti-PR3 (ORG 618, ORG 318) and anti-MPO (ORG 519, ORG 219).

Specifications

Components of the kit