Anti-Neutrophil Cytoplasmic Antibodies (ANCA-Ethanol)
Indirect immunofluorescence test for the qualitative determination of anti-neutrophil cytoplasmic IgG antibodies (ANCA) in serum or plasma on ethanol-fixed human neutrophil granulocytes.
Autoantibodies against cytoplasmic antigens of neutrophil granulocytes (ANCA) are acknowledged serological markers for the diagnosis of systemic vasculitis. Our slides contain ethanol-fixed granulocytes, which facilitate the differentiation of granulomatosis with polyangiitis (GPA; formerly Wegener’s granulomatosis) from eosinophilic granulomatosis with polyangiitis (EGPA; formerly Churg-Strauss syndrome), microscopic polyangiitis (MPA), or Goodpasture syndrome.
A granular immunofluorescence pattern in the cytosol (cANCA) indicates antibodies against proteinase 3 (PR3), which are indicative of GPA. A dense immunofluorescence pattern around the cell nucleus (pANCA) denotes antibodies against myeloperoxidase (MPO), which occur in cases of EGPA or MPA. Our formalin-fixed granulocyte test ORG 892 should be used subsequent to a pANCA positive test result with ethanol-fixed granulocytes to differentiate a true pANCA pattern from an ANA result with a similar perinuclear fluorescence pattern.
In accordance with the “ANCA Consensus Statement” we recommend further serological diagnostic an antigen-specific ELISA test for the individual detection of anti-PR3 (ORG 618, ORG 318) and anti-MPO (ORG 519, ORG 219).
Specifications
Product Number |
ORG 891 |
Tests | 60 (10 slides, 6 wells each) |
Coating |
ethanol fixed neutrophil granulocytes |
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Instruction for Use Revision 3. Earlier versions and additional languages can be found in the document archive.
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Components of the kit
IgG FITC/Evans | |
for human cells and Crithidia luciliae – 3 ml |
Mounting Medium | |
mounting medium – 3 ml |
PBS (10x) | |
phosphate buffer saline (PBS 10x) – 100 ml |
Negative Control | |
Negative Control for IFA – 0.3 ml |
C-ANCA Positive Control | |
antibodies against proteinase 3 – 0.3 ml |
P-ANCA Positive Control | |
antibodies against myeloperoxidase – 0.3 ml |
Slides Anti-Neutrophil Cytoplasmic Antibodies (ANCA-Ethanol) | |
ethanol-fixed human neutrophil granulocytes – 10 slides x 6 wells |
Blotting Paper 1 x 6 | |
filter paper for 6 wells – 10 pcs. |