25-OH Vitamin D₃/D₂

25-OH Vitamin D₃/D₂ is an ELISA test for the quantitative determination of the total concentration of 25-OH vitamin D₃ and 25-OH vitamin D₂ in human serum or plasma (EDTA plasma, heparin plasma, citrate plasma) for the evaluation of vitamin D levels.

A special release reagent developed by ORGENTEC releases the 25-OH-vitamin D₃/D₂ from the vitamin D binding protein complex in the serum – for immediate analysis of the sample. Vitamin D₃ (cholecalciferol) is produced in the skin under irradiation with UV-B rays from the sun. It is metabolized to 25-OH vitamin D₃ in the liver. Vitamin D₂ (ergocalciferol) is mostly produced synthetically and is used as a dietary supplement. It is also converted to its corresponding 25-OH derivative by the liver. This test uses an antibody that binds both forms of vitamin D equally well and with high affinity.

Specifications