Anti-Nuclear Antibodies HEp-2 (ANA-HEp-2)

Indirect immunofluorescence test for the qualitative determination of antinuclear IgG antibodies (ANA) in serum or plasma.

Our cultured Hep-2 cells allow for simultaneous detection of a variety of autoantibodies with a single analytical preparation. Evaluation of different fluorescence patterns provides additional information beyond mere antibody recognition.
Homogeneous distribution of the bound antibodies is indicative of systemic lupus erythematosus (SLE). A speckled pattern is strongly related to SLE, but it may also occur in patients with mixed connective tissue disease, Sjögren's syndrome, polymyositis or scleroderma. Peripheral fluorescence can be observed in patients with connective tissue diseases. A nucleolar pattern has been described in patients with overlapping scleroderma and polymyositis/ dermatomyositis syndromes and with systemic scleroderma, especially for those with renal involvement. In patients with the limited cutaneous form of systemic sclerosis staining of the centromeres can be detected.


Product Number ORG 870
Coating HEp-2


Instruction for Use
Safety Data Sheet
IFA Components
Instruction for Use Revision 3. Earlier versions and additional languages can be found in the document archive.

Components of the kit

Anti-Nuclear Antibodies HEp-2 (ANA-HEp-2) ORG 800 
human cells – 10 slides x 12 wells
ANA-Centromere Positive ControlORG 824
anti-nuclear antibodies (centromere) – 0.3 ml
ANA-HEp-2 Positive ControlORG 825
anti-nuclear antibodies (HEp-2) – 0.3 ml
ANA-Nucleolar Positive ControlORG 827
anti-nuclear antibodies (“nucleolar pattern”) – 0.3 ml
ANA-Speckled Positive ControlORG 828
anti-nuclear antibodies (“speckled pattern”) – 0.3 ml
IgG FITC/EvansORG 837
for human cells and Crithidia luciliae – 3 ml
Mounting MediumORG 842
mounting medium – 3 ml
PBS (10x)ORG 843
phosphate buffer saline (PBS 10x) – 100 ml
Negative ControlORG 844
Negative Control for IFA – 0.3 ml
Blotting Paper 1 x 12ORG 845
filter paper for 12 wells – 10 pcs.
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