25-OH Vitamin D3/D2

25-OH Vitamin D3/D2 is an ELISA-based, automated, in-vitro test system for the quantitative determination of the total concentration of 25-OH vitamin D3 and 25-OH vitamin D2 in human serum or plasma (EDTA plasma, heparin plasma, citrate plasma) for the evaluation of vitamin D levels.

This test uses an antibody that binds both forms of vitamin D equally well and with high affinity. Vitamin D3 (cholecalciferol) is produced in the skin under irradiation with UV-B rays from the sun. It is metabolized to 25-OH vitamin D3 in the liver. Vitamin D2 (ergocalciferol) is mostly produced synthetically and is used as a dietary supplement. It is also converted to its corresponding 25-OH derivative by the liver.
For reliable determination of the total vitamin D concentration, a special sample buffer is used to release the 25-OH vitamin D3 and 25-OH vitamin D2 from the vitamin D binding protein complex in the serum. No separate sample preparation steps are required.

Specifications

Product Number ORG 270
Tests24 Alegria® strips
CalibrationREF!25OHD
Calculationquantitative
Range5 - 200 ng/ml
Coating anti-25-OH Vitamin D3/D2antibodies
ELISA

ORG 570  

Documentation

Instruction for Use
Safety Data Sheet
Product Information

Document Archive
Instruction for Use Revision 5. Earlier versions and additional languages can be found in the document archive.
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